N-2 HYDROXY-4-METHOXYBENZYL (HMB) BACKBONE PROTECTION STRATEGY PREVENTS DOUBLE ASPARTIMIDE FORMATION IN A DIFFICULT PEPTIDE SEQUENCE

被引：39

作者：

PACKMAN, LC

机构：

[1] Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, Tennis Court Road

来源：

TETRAHEDRON LETTERS | 1995年 / 36卷 / 41期

关键词：

D O I：

10.1016/0040-4039(95)01522-1

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Synthesis of an N-terminal, 20 residue fragment of ferredoxin by Fmoc-tert-butyl strategy gave a product in which both aspartic acid residues in an Asp-Gly-Asp-Asn sequence were converted to aspartimide. Resynthesis of the peptide using N-2-hydroxy-4-methoxybenzyl (Hmb) backbone protection at each Asp-Xaa peptide bond resulted in near-complete suppression of this side reaction.

引用

页码：7523 / 7526

页数：4

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