RECOVERY OF PEPTIDES AND PROTEINS FOLLOWING MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY

被引:11
作者
CERPAPOLJAK, A
JENKINS, A
DUNCAN, MW
机构
[1] COOPERAT RES CTR BIOPHARMACEUT RES,DARLINGHURST,NSW 2010,AUSTRALIA
[2] ST VINCENTS HOSP,GARVAN INST MED RES,DARLINGHURST,NSW 2010,AUSTRALIA
[3] UNIV NEW S WALES,BIOMED MASS SPECTROMETRY UNIT,SYDNEY,NSW 2052,AUSTRALIA
关键词
D O I
10.1002/rcm.1290090312
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Only attomole quantities of polypeptide are consumed during analysis by matrix-assisted laser desorption/ionization, despite the need to load picomole quantities of these analytes onto the target. By using radioimmunoassay we have demonstrated that the balance of the material (i.e., effectively all that was loaded onto the slide) can be recovered from the target and remains in an immunoreactive form. The bulk of the material, once recovered from the target, can be examined by other analytical techniques including sodium dodecyl sulphate polyacrylamide gel electrophoresis.
引用
收藏
页码:233 / 239
页数:7
相关论文
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