OXIDATIVE DAMAGE OF MITOCHONDRIA INDUCED BY FE(II)CITRATE IS POTENTIATED BY CA2+ AND INCLUDES LIPID-PEROXIDATION AND ALTERATIONS IN MEMBRANE-PROTEINS

被引：76

作者：

CASTILHO, RF ^{[1
]}

MEINICKE, AR ^{[1
]}

ALMEIDA, AM ^{[1
]}

HERMESLIMA, M ^{[1
]}

VERCESI, AE ^{[1
]}

机构：

[1] UNIV ESTADUAL CAMPINAS, INST BIOL, DEPT BIOQUIM, BR-13084100 CAMPINAS, SP, BRAZIL

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1994年 / 308卷 / 01期

关键词：

D O I：

10.1006/abbi.1994.1022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate. © 1994 Academic Press, Inc.

引用

页码：158 / 163

页数：6

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