METABOLIC PATHWAY FOR 2-KETO-L-GULONIC ACID FORMATION IN GLUCONOBACTER-MELANOGENUS IFO-3293

被引：30

作者：

HOSHINO, T

SUGISAWA, T

TAZOE, M

SHINJOH, M

FUJIWARA, A

机构：

[1] Department of Applied Microbiology, Nippon Roche Research Center, Kamakura, Kanagawa, 247, 200, Kajiwara

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 05期

关键词：

D O I：

10.1080/00021369.1990.10870126

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The l-sorbosone pathway for 2-keto-l-gulonic acid (2KGA) formation from l-sorbose in Gluconobacter melanogenus IFO 3293 was confirmed. l-Sorbose dehydrogenase, which catalyzes the conversion of l-sorbose to l-sorbosone, was found in the membrane fraction of the microorganism, and l-sorbosone dehydrogenase in the cytosol fraction. The latter enzyme requires NAD or NADP as a cofactor. 2KGA was reduced to l-idonic acid by 2KGA reductase, but l -idonic acid was re-oxidized to 2KGA by membrane-bound l-idonate dehydrogenase. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

引用

页码：1211 / 1218

页数：8

共 3 条

[1]

KITAMURA I, 1975, EUR J APPL MICROBIOL, V2, P1

[2] NEW MECHANISMS FOR BIOSYNTHESIS AND METABOLISM OF 2-KETO-L-GULONIC ACID IN BACTERIA [J].

MAKOVER, S ;

RAMSEY, GB ;

VANE, FM ;

WITT, CG ;

WRIGHT, RB .

BIOTECHNOLOGY AND BIOENGINEERING, 1975, 17 (10) :1485-1514

[3] MICROBIAL-PRODUCTION OF 2-KETO-L-GULONIC ACID FROM L-SORBOSE AND D-SORBITOL BY GLUCONOBACTER-MELANOGENUS [J].

SUGISAWA, T ;

HOSHINO, T ;

MASUDA, S ;

NOMURA, S ;

SETOGUCHI, Y ;

TAZOE, M ;

SHINJOH, M ;

SOMEHA, S ;

FUJIWARA, A .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (05) :1201-1209

← 1 →