MICROBIAL-PRODUCTION OF 2-KETO-L-GULONIC ACID FROM L-SORBOSE AND D-SORBITOL BY GLUCONOBACTER-MELANOGENUS

被引：49

作者：

SUGISAWA, T

HOSHINO, T

MASUDA, S

NOMURA, S

SETOGUCHI, Y

TAZOE, M

SHINJOH, M

SOMEHA, S

FUJIWARA, A

机构：

[1] Department of Applied Microbiology, Nippon Roche Research Center, Kamakura, Kanagawa, 247, 200, Kajiwara

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 05期

关键词：

D O I：

10.1080/00021369.1990.10870125

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The strain SPO1 producing 13 g of 2-keto-l-gulonic acid (2KGA) per liter was isolated as a spontaneous mutant of Gluconobacter melanogenus IFO 3293. For the enhancement of 2KGA productivity, we did further strain improvement studies of the mutant. As a result, the mutant U13, producing about 60 g of 2KGA per liter from 100 g of l-sorbose per liter, was obtained. In addition, the mutant Z84, producing about 60 g of 2KGA per liter from 100 g of d-sorbitol per liter, was also obtained. During the fermentation from l-sorbose and d-sorbitol, 5 to 10 g of l-idonic acid per liter was produced as a by-product, but l-idonic acid was converted to 2KGA before the end of fermentation. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

引用

页码：1201 / 1209

页数：9

共 21 条

[1] STUDIES ON REGENERATION MEDIA FOR BACILLUS-SUBTILIS PROTOPLASTS [J].

AKAMATSU, T ;

SEKIGUCHI, J .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (12) :2887-2894

[2] PRODUCTION OF 2-KETO-L-GULONATE, AN INTERMEDIATE IN L-ASCORBATE SYNTHESIS, BY A GENETICALLY MODIFIED ERWINIA-HERBICOLA [J].

ANDERSON, S ;

MARKS, CB ;

LAZARUS, R ;

MILLER, J ;

STAFFORD, K ;

SEYMOUR, J ;

LIGHT, D ;

RASTETTER, W ;

ESTELL, D .

SCIENCE, 1985, 230 (4722) :144-149

[3]

CHIBATA I, 1974, Patent No. 39838

[4]

Huang H. T., 1962, US Patent, Patent No. [3,043,749, 3043749]

[5] 2-KETO-L-GULONIC ACID FERMENTATION .I. PAPER CHROMATOGRAPHIC CHARACTERIZATION OF METABOLIC PRODUCTS FROM SORBITOL AND L-SORBOSE BY VARIOUS BACTERIA [J].

ISONO, M ;

NAKANISHI, I ;

SASAJIMA, KI ;

MOTIZUKI, K ;

KANZAKI, T ;

OKAZAKI, H ;

YOSHINO, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1968, 32 (04) :424-+

[6] 2-KETO-L-GULONIC ACID FERMENTATION .4. L-SORBOSE METABOLISM IN PSEUDOMONAS-AERUGINOSA [J].

KANZAKI, T ;

OKAZAKI, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1970, 34 (03) :432-&

[7]

KITAMURA I, 1975, EUR J APPL MICROBIOL, V2, P1

[8]

Kulhanek M, 1970, Adv Appl Microbiol, V12, P11, DOI 10.1016/S0065-2164(08)70581-1

[9]

LIN YG, 1980, ACTA MICROBIOLOGICA, V20, P246

[10] NEW MECHANISMS FOR BIOSYNTHESIS AND METABOLISM OF 2-KETO-L-GULONIC ACID IN BACTERIA [J].

MAKOVER, S ;

RAMSEY, GB ;

VANE, FM ;

WITT, CG ;

WRIGHT, RB .

BIOTECHNOLOGY AND BIOENGINEERING, 1975, 17 (10) :1485-1514

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