DISSOCIATED CELL-SUSPENSIONS OF CARCINUS-MAENAS Y-ORGANS AS A TOOL TO STUDY ECDYSTEROID PRODUCTION AND ITS REGULATION

被引：24

作者：

TOULLEC, JY ^{[1
]}

DAUPHINVILLEMANT, C ^{[1
]}

机构：

[1] ECOLE NORMALE SUPER,BIOCHIM & PHYSIOL DEV LAB,CNRS,URA 686,F-75230 PARIS 05,FRANCE

来源：

EXPERIENTIA | 1994年 / 50卷 / 02期

关键词：

ECDYSTEROID BIOSYNTHESIS; NEUROPEPTIDE REGULATION; Y-ORGANS; CRUSTACEA;

D O I：

10.1007/BF01984955

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In vitro ecdysteroid production by dissociated Y-organ cells of the shore crab Carcinus maenas was characterized during short-term incubations. Under optimized conditions (M199 adjusted to crab osmolality and with the addition of 10% foetal calf serum), ecdysteroid production by dispersed cells increased linearly during 4-hour incubations, with little intra-assay variation. 25-deoxyecdysone was mainly produced. Purified Carcinus molt inhibiting hormone (CamMIH) produced a dose-dependent inhibition of ecdysteroid production by dispersed cells. The cells were about 50 times more sensitive than whole glands to MIH. Other structurally-related peptides were tested.

引用

页码：153 / 158

页数：6

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