IMMUNOELECTROPHORETIC IDENTIFICATION AND PURIFICATION OF HERPES-SIMPLEX VIRUS-ANTIGENS RELEASED FROM INFECTED-CELLS IN TISSUE-CULTURE

被引：23

作者：

NORRILD, B ^{[1
]}

VESTERGAARD, BF ^{[1
]}

机构：

[1] UNIV COPENHAGEN,INST MED MICROBIOL,COPENHAGEN,DENMARK

来源：

INTERVIROLOGY | 1979年 / 11卷 / 02期

关键词：

D O I：

10.1159/000149020

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Proteins released from herpes simplex virus type 1 (HSV-1)- and type 2 (HSV-2)-infected HEp-2 cells have been characterized by the crossed immunoelectrophoretic technique. Both HSV type-common and type-specific antigens were found in the tissue culture medium 24 hr after infection. Antigen Ag-6, an HSV-1-specific antigen, was found in high concentration in the medium as compared to other HSV antigens released from HSV-1-infected cells. The HSV-2-specific antigens, Ag-4 and Ag-1, were released in molecular modifications with altered electrophoretic mobility as compared to their cellular counterparts. Purification of HSV antigens was performed by ion-exchange chromatography, and an HSV type-common antigen, Ag-11, and an HSV-2-specific antigen, Ag-4A, were isolated.

引用

页码：104 / 110

页数：7