SOLUBILIZATION OF RECEPTOR FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE FROM BOVINE BRAIN

被引：20

作者：

MASUDA, Y

OHTAKI, T

KITADA, C

TSUDA, M

ARIMURA, A

FUJINO, M

机构：

[1] TAKEDA CHEM IND LTD,TSUKUBA RES LABS,WADAI 7,TSUKUBA,IBARAKI 30042,JAPAN

[2] TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,BELLE CHASSE,LA 70037

[3] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70112

[4] TULANE UNIV,SCH MED,DEPT ANAT,NEW ORLEANS,LA 70112

[5] TULANE UNIV,SCH MED,DEPT PHYSIOL,NEW ORLEANS,LA 70112

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90732-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A receptor for pituitary adenylate cyclase activating polypeptide (PACAP) was solubilized from bovine brain membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Saturable binding experiments using [125I]PACAP27 revealed that the solubilized extract contained a single class of binding sites with a Kd of 200 pM and a Bmax of 0.9 pmol/mg protein. The Kd did not change significantly after the solubilization. Competitive binding experiments confirmed that the solubilized receptor retained a specificity for PACAP. Thus, the solubilization of the receptor in an active form was successfully achieved by the present procedure. © 1990.

引用

页码：709 / 714

页数：6

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