THE STRUCTURE OF THE HUMAN LIVER-TYPE PHOSPHOFRUCTOKINASE GENE

被引：35

作者：

ELSON, A ^{[1
]}

LEVANON, D ^{[1
]}

BRANDEIS, M ^{[1
]}

DAFNI, N ^{[1
]}

BERNSTEIN, Y ^{[1
]}

DANCIGER, E ^{[1
]}

GRONER, Y ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT MOLEC GENET & VIROL,IL-76100 REHOVOT,ISRAEL

来源：

GENOMICS | 1990年 / 7卷 / 01期

关键词：

D O I：

10.1016/0888-7543(90)90517-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have isolated the gene for the human liver-type phosphofructokinase, from upstream to the 5′ mRNA terminus to beyond the polyadenylation site. The gene is at least 28 kb long and is divided into 22 exons; it contains conventional splice-junction sequences and one polyadenylation signal. Exons and introns are quite rich in G and C residues; some 60% of all nucleotides are either G or C. Five possible sites of polymorphism have been found. The gene structure reveals no signs of internal similarities despite protein sequence evidence which suggests that the PFK molecule is divided into two similar halves. The structure and organization of the human liver-type PFK gene are shown to be extremely similar to those of the rabbit muscle-type PFK. © 1990.

引用

页码：47 / 56

页数：10

共 59 条

[21]

LEE CP, 1987, J BIOL CHEM, V262, P4195

[22] GENOMIC CLONES OF THE HUMAN LIVER-TYPE PHOSPHOFRUCTOKINASE [J].