THE FUNCTION OF SELENOCYSTEINE SYNTHASE AND SELB IN THE SYNTHESIS AND INCORPORATION OF SELENOCYSTEINE

被引：33

作者：

FORCHHAMMER, K ^{[1
]}

BOESMILLER, K ^{[1
]}

BOCK, A ^{[1
]}

机构：

[1] UNIV MUNICH,LEHRSTUHL MIKROBIOL,MARIA WARD STR 1A,W-8000 MUNICH 19,GERMANY

来源：

BIOCHIMIE | 1991年 / 73卷 / 12期

关键词：

SELENOCYSTEINE SYNTHASE; TRANSFER RNA-BINDING; ELONGATION FACTOR SELB; UGA TRANSLATION;

D O I：

10.1016/0300-9084(91)90181-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The selAB operon codes for the proteins selenocysteine synthase and SELB which catalyse the synthesis and co-translational insertion of selenocysteine into protein. This communication deals with the biochemical characterisation of these proteins and in particular with their specific interaction with the selenocysteine-incorporating tRNA(Sec). Selenocysteine synthase catalyses the synthesis of selenocysteyl-tRNA(Sec) from seryl-tRNA(Sec) in a pyridoxal phosphate-dependent reaction mechanism. The enzyme specifically recognizes the tRNA(Sec) molecule; a cooperative interaction between the tRNA binding site and the catalytically active pyridoxal phosphate site is suggested. SELB is an EF-Tu-like protein which specifically complexes selenocysteyl-tRNA(Sec). Interaction with the selenol group of the side chain of the aminoacylated residue is a prerequisite for the formation of a stable SELB.tRNA complex. Mechanistically, this provides the biochemical basis for the exclusive selection of selenocysteyl-tRNA(Sec) in the decoding step of a selenocysteine-specific UGA triplet.

引用

页码：1481 / 1486

页数：6

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