A NOVEL PEPTIDE FROM FUNNEL-WEB SPIDER VENOM, OMEGA-AGA-TK, SELECTIVELY BLOCKS P-TYPE CALCIUM CHANNELS

被引：61

作者：

TERAMOTO, T

KUWADA, M

NIIDOME, T

SAWADA, K

NISHIZAWA, Y

KATAYAMA, K

机构：

[1] Eisai Tsukuba Research Laboratories, Tsukuba-shi, Ibaraki 300-26

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2225

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the course of purification of ω-Aga-IVA, a specific P-type calcium channel blocker, from the venom of Agelenopsis aperta we discovered a novel peptide. This peptide, named ω-agatoxin Tsukuba (ω-Aga-TK), also blocked P-type channels and was twelve times more abundant in the venom than ω-Aga-IVA. ω-Aga-TK was purified to homogeneity by a two-step reverse-phase HPLC procedure. Its amino acid sequence is 71% identical to that of ω-Aga-IVA. ω-Aga-TK has a negatively charged N-terminus, whereas α-Aga-IVAA has a positively charged one. Electrophysiological data indicate that ω-Aga-TK is a potent and selective inhibitor of P-type channels. © 1993 Academic Press, Inc.

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页码：134 / 140

页数：7

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