PRELIMINARY FRACTIONATION OF TIGER RATTLESNAKE (CROTALUS-TIGRIS) VENOM

被引：10

作者：

WEINSTEIN, SA ^{[1
]}

SMITH, LA ^{[1
]}

机构：

[1] USA,MED RES INST INFECT DIS,DEPT TOXINOL,DIV PATHOL,FREDERICK,MD 21701

来源：

TOXICON | 1990年 / 28卷 / 12期

关键词：

D O I：

10.1016/0041-0101(90)90158-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

S. A. Weinstein and L. A. Smith. Preliminary fractionation of tiger rattlesnake (Crotalus tigris) venom. Toxicon 28, 1447-1455, 1990.-Tiger rattlesnake (Crotalus tigris) venom was fractioned by using fast protein liquid chromatography (FPLC). The crude venom had low protease activity, lacked hemolytic activity and had an i.p. ld50 of 0.070 mg/kg for mice. Lethal fractions obtained by anion and cation exchange were examined for antigenic identity with crotoxin and Mojave toxin. Four toxins were obtained by anion exchange chromatography which showed immunoidentity with these toxins, and one fraction caused rear limb paresis in mice. A lethal toxin (about 10% of total venom protein) purified further with Superose-12 FPLC (molecular sieve) had an i.p. ld50 of 0.050 mg/kg for mice, reacted strongly with anti-crotoxin and anti-Mojave toxin antiserum in ELISA and immunoelectrophoresis. This toxin also showed complete immunoidentity with crotoxin and Mojave toxin in immunodiffusion assays with anti-crotoxin antiserum. The results indicated the presence of crotoxin and/or Mojave toxin isoforms in this venom. Although this species has a low venom yield (average 10 mg per snake), the venom is highly toxic and contains high concentrations of several neurotoxic isotoxins. © 1990.

引用

页码：1447 / 1455

页数：9

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