OCCURRENCE AND POSTMORTEM GENERATION OF ANANDAMIDE AND OTHER LONG-CHAIN N-ACYLETHANOLAMINES IN MAMMALIAN BRAIN

被引：192

作者：

SCHMID, PC ^{[1
]}

KREBSBACH, RJ ^{[1
]}

PERRY, SR ^{[1
]}

DETTMER, TM ^{[1
]}

MAASSON, JL ^{[1
]}

SCHMID, HHO ^{[1
]}

机构：

[1] UNIV MINNESOTA, HORMEL INST, AUSTIN, MN 55912 USA

来源：

FEBS LETTERS | 1995年 / 375卷 / 1-2期

关键词：

N-ACYLETHANOLAMINE; ANANDAMIDE; ARACHIDONIC ACID; PIG BRAIN; SHEEP BRAIN; COW BRAIN;

D O I：

10.1016/0014-5793(95)01194-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long-chain N-acylethanolamines (NAEs) were assayed in pig, sheep and cow brain by gas chromatography/mass spectrometry of their ter tert.-butyldimethylsilyl derivatives in the presence of deuterium-labeled internal standards, Immediately after death, total NAEs ranged from about 2.7 mu g/g wet weight (sheep, cow) to 6.5 mu g/g wet weight (pig) and consisted almost exclusively (99%) of saturated and monounsaturated species, Anandamide (20:4n-6 NAE) comprised about 1% of total NAE in pig and cow brain, but was absent in freshly extracted sheep brain, When pig brain was analysed after 0.5, 1, 3, 4 and 23 h at ambient temperature, NAE levels were found to increase substantially over the entire time period with 20:4n-6 NAE formation exhibiting a time lag compared to that of saturated and monounsaturated NAEs.

引用

页码：117 / 120

页数：4

共 32 条

[1]

BACHUR NR, 1966, J BIOL CHEM, V241, P1308

[2] EFFECTS OF ISCHEMIA AND ELECTROCONVULSIVE SHOCK ON FREE FATTY ACID POOL IN BRAIN [J].