OXIDATIVE MODIFICATION OF LP(A) CAUSES CHANGES IN THE STRUCTURE AND BIOLOGICAL PROPERTIES OF APO(A)

被引：25

作者：

NARUSZEWICZE, M ^{[1
]}

GIROUX, LM ^{[1
]}

DAVIGNON, J ^{[1
]}

机构：

[1] CLIN RES INST MONTREAL, HYPERLIPIDEMIA & ATHEROSCLEROSIS RES GRP, MONTREAL H2W 1R7, PQ, CANADA

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1994年 / 67-8卷

基金：

英国医学研究理事会;

关键词：

U937; CELLS; PLASMINOGEN BINDING; MACROPHAGE SCAVENGER RECEPTOR; LP(A); OXIDATION;

D O I：

10.1016/0009-3084(94)90136-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously showed that oxidative modification converted Lp(a) particles to a form readily recognized by macrophage scavenger receptor(s). This was mediated in part by apo(a) changes indicated by a stronger negative charge protein fragmentation and increased immunoreactivity to monoclonal antibody K07, which also cross-reacted with plasminogen (Pg). The present study demonstrated that the expression of K07 and K09 epitopes of apo(a) increased markedly during time-dependent oxidative modification of Lp(a) by copper ions. Incubation of oxidized Lp(a) with the monocytoid U937 cell line showed that these particles competed more effectively with I-125-type 2 Pg binding to specific cell surface receptors than native Lp(a). This study suggests that oxidative Lp(a) modification causes significant changes in apo(a) conformation, resulting in the enhanced interaction of these particles with macrophage scavenger receptors and Pg binding sites.

引用

页码：167 / 174

页数：8

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