CHARACTERIZATION OF MENGO-VIRUS NEUTRALIZATION EPITOPES .2. INFECTION OF MICE WITH AN ATTENUATED VIRUS

被引:9
作者
KOBASA, D
MULVEY, M
LEE, JS
SCRABA, DG
机构
[1] UNIV ALBERTA,DEPT BIOCHEM,EDMONTON,AB T6G 2H7,CANADA
[2] UNIV SASKATCHEWAN,DEPT BIOCHEM,SASKATOON,SK S7N 5E5,CANADA
基金
英国医学研究理事会;
关键词
D O I
10.1006/viro.1995.9948
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A panel of five neutralizing monoclonal antibodies was generated from mice immunized with an attenuated strain of Mengo virus. Four of the antibodies were used to select mutants of Mengo virus which were able to escape neutralization by the selecting antibody, but it was not possible to select mutants which could escape neutralization by the fifth antibody. The capsid coding region of the RNA genome of each mutant was directly sequenced to identify the mutation(s) responsible for the neutralization escape phenotype. These results are compared to those of a previous study in which immunogenic determinants recognized by neutralizing antibodies generated against pentameric capsid subunits were located on the external surface of the Mengo virion. We have confirmed the existence of the previously identified immunogenic determinant in VP3 (site 2) as well as an immunodominant determinant in VP2 (site 1). Two previously uncharacterized determinants, located in surface loops of VP1 (sites 3 and 4), were also identified. None of the mutations conferring the neutralization escape phenotype was found near the surface depressions on the virion which are believed to be the receptor binding sites. (C) 1995 Academic Press, Inc.
引用
收藏
页码:118 / 127
页数:10
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