INVOLVEMENT OF SIMIAN VIRUS-40 (SV40) SMALL T-ANTIGEN IN TRANSACTIVATION OF SV40 EARLY AND LATE PROMOTERS

被引:18
作者
BIKEL, I
LOEKEN, MR
机构
[1] JOSLIN DIABET CTR,1 JOSLIN PL,BOSTON,MA 02215
[2] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115
关键词
D O I
10.1128/JVI.66.3.1489-1494.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have previously found that simian virus 40 (SV40) small t antigen (small t) can trans activate the E2A and VA-I genes of adenovirus in plasmid DNA-transfected cells (M.R. Loeken, I. Bikel, D.M. Livingston, and J. Brady, Cell 55:1171-1177, 1988). To determine whether trans activation by small t might be involved in the SV40 productive infection cycle, we examined the effects of cotransfecting plasmids encoding small t with plasmids containing the chloramphenicol acetyltransferase (CAT) gene linked to the SV40 early or late promoter. Small t increased three- to fivefold the expression of a CAT plasmid linked to the SV40 early promoter and enhancer. Small t expression had no effect by itself on CAT activity directed by the SV40 late promoter, but small t enhanced the effect of a suboptimal concentration of a plasmid expressing large T up to 10-fold. When the concentration of the plasmid expressing large T was increased to a level at which large T alone stimulated the late promoter ninefold, the enhancement by small t was only twofold. The effects of small t on both the SV40 early and late promoters depended on sequences within the small t-unique domain, since a plasmid expressing only the first 82 amino acids common to both large T and small t was inactive. The effects of small t on early- and late-promoter-directed CAT enzyme activity was reflected in increased CAT mRNA as measured by S1 analysis. These results suggest that SV40 small t may play a role in viral infection by increasing transcription from the early promoter and from the late promoter at times when large T levels are low.
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页码:1489 / 1494
页数:6
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