SIMULTANEOUS DETERMINATION OF INORGANIC CATIONS AND ANIONS IN CAPILLARY ZONE ELECTROPHORESIS (CZE) WITH INDIRECT FLUORESCENCE DETECTION

An instrumental configuration for the simultaneous determination of inorganic cations and anions in capillary zone electrophoresis (CZE) is described. The sample is injected into the central part of the capillary by hydrostatic injection. Indirect fluorescence detection is used. The electrolyte system consists of 2,5-dihydrobenzoate as fluorescent anion and cerium(III) as fluorescent cation. This method allows the analysis of both cations and anions without changing the electrolyte system in less than five minutes.