8-VINYL REDUCTION AND CHLOROPHYLL A BIOSYNTHESIS IN HIGHER-PLANTS

被引：31

作者：

WHYTE, BJ ^{[1
]}

GRIFFITHS, WT ^{[1
]}

机构：

[1] UNIV BRISTOL, SCH MED SCI, DEPT BIOCHEM, BRISTOL BS8 1TD, AVON, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1993年 / 291卷

关键词：

D O I：

10.1042/bj2910939

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique involving solid-phase extractions and polyethylene h.p.l.c suitable for the routine compositional analysis of the total protochlorophyllide pool of plants is described. The resynthesis kinetics of the individual components of the pool have been studied in briefly illuminated etiolated tissue of wheat (Triticum aestivum) and cucumber (Cucumis sativus) during subsequent redarkening. The data are interpreted in terms of a precursor-product relationship between the di- and mono-vinyl analogues of protochlorophyllide during their reaccumulation in darkness. The interconversion is assumed to be catalysed by an 8-vinyl reductase, which shows greater activity in wheat than in cucumber. Analyses of the redox state of the nicotinamide nucleotide of the pool during the process are compatible with NADPH as the cofactor of the putative reductase.

引用

页码：939 / 944

页数：6

共 31 条

[11] REDUCTION OF VINYL SIDE-CHAINS OF MG-PROTOPORPHYRIN-IX, MONOMETHYL ESTER IN-VITRO [J].

ELLSWORTH, RK ;

HSING, AS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 313 (01) :119-129

[12]

ELLSWORTH RK, 1972, CHEM PLANT PIGMENTS, V111, P85

[13] DEFECTIVE SYNTHESIS OF PORPHYRINS IN BARLEY PLASTIDS CAUSED BY MUTATION IN NUCLEAR GENES [J].

GOUGH, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 286 (01) :36-&

[14]

GRANICK S, 1949, HARVEY LECT, V44, P220

[15] SUBSTRATE-SPECIFICITY STUDIES ON PROTOCHLOROPHYLLIDE REDUCTASE IN BARLEY (HORDEUM-VULGARE) ETIOPLAST MEMBRANES [J].

GRIFFITHS, WT .

BIOCHEMICAL JOURNAL, 1980, 186 (01) :267-278

[16] RECONSTITUTION OF CHLOROPHYLLIDE FORMATION BY ISOLATED ETIOPLAST MEMBRANES [J].

GRIFFITHS, WT .

BIOCHEMICAL JOURNAL, 1978, 174 (03) :681-692

[17] SEPARATION OF MONOVINYL AND DIVINYL PROTOCHLOROPHYLLIDES AND CHLOROPHYLLIDES FROM ETIOLATED AND PHOTOTRANSFORMED CUCUMBER COTYLEDONS [J].

HANAMOTO, CM ;

CASTELFRANCO, PA .

PLANT PHYSIOLOGY, 1983, 73 (01) :79-81

[18] COMPARTMENTATION AND REDUCTION OF PYRIDINE NUCLEOTIDES IN RELATION TO PHOTOSYNTHESIS [J].

HEBER, UW ;

SANTARIUS, KA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 109 (02) :390-+

[19]

LOEFFLER JE, 1955, YB CARNEGIE I WASHIN, V54, P159

[20] PHOTODYNAMIC HERBICIDES - RECENT DEVELOPMENTS AND MOLECULAR-BASIS OF SELECTIVITY [J].

REBEIZ, CA ;

MONTAZERZOUHOOR, A ;

MAYASICH, JM ;

TRIPATHY, BC ;

WU, SM ;

REBEIZ, CC .

CRC CRITICAL REVIEWS IN PLANT SCIENCES, 1988, 6 (04) :385-436

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