NONCOVALENT INTERACTION BETWEEN POLY(ADP-RIBOSE) AND CELLULAR PROTEINS - AN APPLICATION OF A POLY(ADP-RIBOSE) WESTERN BLOTTING METHOD TO DETECT POLY(ADP-RIBOSE) BINDING ON PROTEIN-BLOTTED FILTER

被引：20

作者：

NOZAKI, T

MASUTANI, M

AKAGAWA, T

SUGIMURA, T

ESUMI, H

机构：

[1] NATL CANC CTR,RES INST,DIV BIOCHEM,CHUO KU,TOKYO 104,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT ORAL & MAXILLOFACIAL SURG,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a sensitive method for the detection of interactions between poly(ADP-ribose) and proteins. Proteins were blotted onto nitrocellulose filters and incubated with 32P-labeled poly(ADP-ribose). Purified core histones and poly(ADP-ribose) polymerase were found to bind poly(ADP-ribose) polymer. Blots of HeLa cell protein extracts revealed a 48 kDa protein and several others of smaller than 35 kDa likewise bound the polymers even at high salt concentrations. Those proteins, along with a 69 kDa protein, also showed resistance to competitor DNA. Polymer binding of aforesaid HeLa extract proteins was restricted to polymers above 20 residues in length. Thus poly(ADP-ribose)-protein affinities were polymer-length dependent. © 1994 Academic Press. All rights reserved.

引用

页码：45 / 51

页数：7

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