IDENTIFICATION OF 2 OPAQUE2 MODIFIER LOCI IN QUALITY-PROTEIN-MAIZE

被引：48

作者：

LOPES, MA ^{[1
]}

TAKASAKI, K ^{[1
]}

BOSTWICK, DE ^{[1
]}

HELENTJARIS, T ^{[1
]}

LARKINS, BA ^{[1
]}

机构：

[1] UNIV ARIZONA, DEPT PLANT SCI, TUCSON, AZ 85721 USA

来源：

MOLECULAR AND GENERAL GENETICS | 1995年 / 247卷 / 05期

关键词：

GAMMA-ZEIN; ENDOSPERM; STORAGE PROTEIN; GENE MAPPING;

D O I：

10.1007/BF00290352

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic modifiers of opaque2 convert the soft, starchy endosperm of opaque2 maize mutants to a hard, vitreous phenotype, while maintaining the enhanced lysine content of the grain. Genetic analysis of F2 segregating seeds from crosses of opaque2 by modified opaque2 genotypes indicated that the modifiers are complex traits that act codominantly. We developed two different segregating F2 populations and mapped the modifier loci by restriction fragment length polymorphism (RFLP) analysis. A relationship was found between formation of vitreous endosperm and the locus encoding the gamma-zein storage protein, which maps near the centromere of chromosome 7. Endosperm modification was consistently associated with the presence of two rather than one gamma-zein gene at this locus. A second modifier locus was mapped near the telomere of chromosome 7L.

引用

页码：603 / 613

页数：11