SYNAPTOTAGMIN IS ENDOGENOUSLY PHOSPHORYLATED BY CA-2+ CALMODULIN PROTEIN KINASE-II IN SYNAPTIC VESICLES

被引：63

作者：

POPOLI, M ^{[1
]}

机构：

[1] UNIV NAPLES SCH MED,NEUROCHEM LAB,I-80131 NAPLES,ITALY

来源：

FEBS LETTERS | 1993年 / 317卷 / 1-2期

关键词：

P65-SYNAPTOTAGMIN; SYNAPTIC VESICLE; NEUROTRANSMITTER RELEASE; PROTEIN PHOSPHORYLATION; CALCIUM CALMODULIN KINASE-II;

D O I：

10.1016/0014-5793(93)81496-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytoplasmic domain of synaptotagmin (a synaptic vesicle-specific protein) has a high degree of homology with the Ca2+-phospholipid binding domain of protein kinase C. The Ca2+-phospholipid binding activity of synaptotagmin has been implicated in the docking and fusion of synaptic vesicles with the presynaptic membrane during Ca2+-induced exocytosis. The protein sequence contains potential phosphorylation sites for various protein kinases which could modulate its binding activity. At present there is no clear evidence that the protein is endogenously phosphorylated in intact vesicles. Here it is reported that phospho-synaptotagmin was immunoprecipitated from endogenously phosphorylated synaptic vesicles. The conditions used indicate that synaptotagmin, as synapsin I, is phosphorylated by Ca2+/calmodulin-dependent protein kinase II.

引用

页码：85 / 88

页数：4

共 17 条

[1] HIGHLY SEQUENTIAL BINDING OF PROTEIN-KINASE-C AND RELATED PROTEINS TO MEMBRANES [J].

BAZZI, MD ;

NELSESTUEN, GL .

BIOCHEMISTRY, 1991, 30 (32) :7970-7977

[2] SYNAPTIC VESICLE-ASSOCIATED CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE-II IS A BINDING-PROTEIN FOR SYNAPSIN-I [J].

BENFENATI, F ;

VALTORTA, F ;

RUBENSTEIN, JL ;

GORELICK, FS ;

GREENGARD, P ;

CZERNIK, AJ .

NATURE, 1992, 359 (6394) :417-420

[3] SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES [J].

BENNETT, MK ;

CALAKOS, N ;

SCHELLER, RH .

SCIENCE, 1992, 257 (5067) :255-259

[4] SYNAPTOTAGMIN - A CALCIUM SENSOR ON THE SYNAPTIC VESICLE SURFACE [J].

BROSE, N ;

PETRENKO, AG ;

SUDHOF, TC ;

JAHN, R .

SCIENCE, 1992, 256 (5059) :1021-1025

[5] AMINO-ACID-SEQUENCES SURROUNDING THE CAMP-DEPENDENT AND CALCIUM CALMODULIN-DEPENDENT PHOSPHORYLATION SITES IN RAT AND BOVINE SYNAPSIN-I [J].

CZERNIK, AJ ;

PANG, DT ;

GREENGARD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7518-7522

[6] MICROINJECTED ANTIBODIES AGAINST THE CYTOPLASMIC DOMAIN OF VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN BLOCK ITS TRANSPORT TO THE CELL-SURFACE [J].

KREIS, TE .

EMBO JOURNAL, 1986, 5 (05) :931-941

[7] THE SYNAPTIC VESICLE PROTEIN SYNAPTOTAGMIN ASSOCIATES WITH CALCIUM CHANNELS AND IS A PUTATIVE LAMBERT-EATON MYASTHENIC SYNDROME ANTIGEN [J].

LEVEQUE, C ;

HOSHINO, T ;

DAVID, P ;

SHOJIKASAI, Y ;

LEYS, K ;

OMORI, A ;

LANG, B ;

ELFAR, O ;

SATO, K ;

MARTINMOUTOT, N ;

NEWSOMDAVIS, J ;

TAKAHASHI, M ;

SEAGAR, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3625-3629

[8] IDENTIFICATION OF A SYNAPTIC VESICLE-SPECIFIC MEMBRANE-PROTEIN WITH A WIDE DISTRIBUTION IN NEURONAL AND NEUROSECRETORY TISSUE [J].

MATTHEW, WD ;

TSAVALER, L ;

REICHARDT, LF .

JOURNAL OF CELL BIOLOGY, 1981, 91 (01) :257-269

[9] CALCIUM CALMODULIN-DEPENDENT PROTEIN KINASE-II INCREASES GLUTAMATE AND NORADRENALINE RELEASE FROM SYNAPTOSOMES [J].

NICHOLS, RA ;

SIHRA, TS ;

CZERNIK, AJ ;

NAIRN, AC ;

GREENGARD, P .

NATURE, 1990, 343 (6259) :647-651

[10]

PERIN MS, 1991, J BIOL CHEM, V266, P623

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