INDUCTION BY ZINC OF SPECIFIC METALLOTHIONEIN ISOFORMS IN HUMAN MONOCYTES

被引：47

作者：

PAUWELS, M

VANWEYENBERGH, J

SOUMILLION, A

PROOST, P

DELEY, M

机构：

[1] KATHOLIEKE UNIV LEUVEN, BIOCHEM LAB, B-3000 LOUVAIN, BELGIUM

[2] KATHOLIEKE UNIV LEUVEN, REGA INST, LOUVAIN, BELGIUM

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 220卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1994.tb18603.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A low-molecular-mass zinc-containing protein was isolated by gel permeation and anion-exchange chromatography of lysates of human monocytes induced with zinc acetate. Characterization by sodium dodecyl sulphate/polyacrylamide gel electrophoresis and amino acid sequencing identified the two major charge-separable fractions and an occasionally occurring third fraction as metallothionein-1, metallothionein-2 and metallothionein-0, respectively. Metallothionein-1 was shown to consist of a mixture of isoforms, confirmed as metallothionein-1e, metallothionein-1g and metallothionein-11 by comparison with cDNA sequences obtained by screening a human monocyte cDNA library. We can find no previous observation in the literature of metallothionein-1g at both the protein and RNA level in a non-tumour cell, and of metallothionein-0 in a non-fetal cell or tissue. Since isoform-specific polymerase-chain-reaction amplification showed the presence of metallothionein-0 mRNA in zinc-induced but not in untreated monocytes, these cells can be used as an in vitro system to investigate the expression of this previously considered fetal isoform.

引用

页码：105 / 110

页数：6

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