CAPILLARY ELECTROPHORETIC ANALYSIS OF FLAVONOIDS

被引：61

作者：

SEITZ, U

OEFNER, PJ

NATHAKARNKITKOOL, S

POPP, M

BONN, GK

机构：

[1] UNIV INNSBRUCK,INST RADIOCHEM,A-6020 INNSBRUCK,AUSTRIA

[2] PLANTAMED,NEUMARKT,POLAND

[3] UNIV LINZ,DEPT ANALYT CHEM,LINZ,AUSTRIA

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 1-2期

关键词：

D O I：

10.1002/elps.1150130107

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Combining the effects of electrophoresis and electroendosmosis,flavonoids were separated in less than ten minutes in a fused silica capillary tube with a borate buffer adjusted to pH 10. An increase in the concentration of borate from 0.1 to 0.2 M resulted in longer migration times due to a decrease in electroosmotic flow, but also in improved selectivity and higher resolution of flavonoids. The calibration curve of rutin showed a detection limit of 0.02 mg/mL and linearity over its pharmaceutical concentration range. Using an internal standard of known concentration, the content of rutin in a methanolic extract of Sambuci flos could be determined with a coefficient of variation as small as 3.8% by the molar ratio - peak area ratio method.

引用

页码：35 / 38

页数：4