SINGLE-BASE POLYMORPHISM LINKED TO THE ATAXIA-TELANGIECTASIA LOCUS IS DETECTED BY MISMATCH PCR

被引：4

作者：

ATHMA, P

FIDAHUSEIN, N

SWIFT, M

机构：

[1] Division of Human Molecular Genetics, Department of Pediatrics, New York Medical College, Valhalla, NY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1753

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymorphic site at D11S384, which shows zero recombination with the ataxia-telangiectasia (A-T) locus, was originally detected by Mutation Detection Enhancement Gel and Denaturing Gradient Gel Electrophoresis. In order to increase the throughput and decrease the complexity of the assays, we developed a site directed mutagenesis using primers with mismatched 3'ends, followed by restriction digestion, as a rapid, nonradioactive method for detecting polymorphisms/mutations. (C) 1995 Academic Press, Inc.

引用

页码：982 / 986

页数：5

共 12 条

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