THE EFFECTIVE DIFFUSIVE PERMEABILITY OF A NONREACTING SOLUTE IN MICROBIAL CELL AGGREGATES

被引：84

作者：

LIBICKI, SB ^{[1
]}

SALMON, PM ^{[1
]}

ROBERTSON, CR ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT CHEM ENGN,STANFORD,CA 94305

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1988年 / 32卷 / 01期

关键词：

BACTERIOLOGY - BIOMASS - CELL CULTURE - Immobilization - FLOW OF FLUIDS - Two Phase - MICROSCOPIC EXAMINATION - Transmission Electron Microscopy;

D O I：

10.1002/bit.260320111

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aggregates of Escherichia coli confined within hollow-fiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide within the cell aggregates was calculated from measurements of the tracer flux through the aggregates. Estimates of the hydraulic permeability were also obtained for the aggregates that were grown in place. The effective diffusive permeability was found to decrease with increasing cell volume fraction to a value, for aggregates comprising 95% cells, of ca. 30% that obtained for cell-free buffer solution. The dependence on the cell volume fraction was described adequately by the well-known Hashin-Shtrikman bounds for a two-phase medium. The transport properties of aggregates cultivated in place were not significantly different from those of aggregates prepared by centrifugation.

引用

页码：68 / 85

页数：18

共 69 条

[1] OXYGEN-SUPPLY TO IMMOBILIZED CELLS .5. THEORETICAL CALCULATIONS AND EXPERIMENTAL-DATA FOR THE OXIDATION OF GLYCEROL BY IMMOBILIZED GLUCONOBACTER-OXYDANS CELLS WITH OXYGEN OR P-BENZOQUINONE AS ELECTRON-ACCEPTOR [J].