DETERMINATION OF 7-METHYLGUANOSINE-CONTAINING 5'-TERMINI OF MESSENGER RIBONUCLEIC-ACID BY NAB[H-3]4 LABELING AND HIGH-PERFORMANCE LIQUID ANION-EXCHANGE CHROMATOGRAPHY

被引：16

作者：

BRETER, HJ ^{[1
]}

MALEK, LT ^{[1
]}

HELLMANN, GM ^{[1
]}

RHOADS, RE ^{[1
]}

机构：

[1] UNIV KENTUCKY,COLL MED,DEPT BIOCHEM,LEXINGTON,KY 40536

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90712-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the determination of 5′-terminal methylated (cap) structures in unlabeled mRNA based on oxidation with NaIO4, reduction with NaB[3H]4, cleavage with P1 nuclease, and separation on a strong anion-exchange resin by high-performance liquid chromatography (hplc). Model compounds (cap 1 dinucleotides) were used to show that no structural alteration other than cleavage of the ribose ring of 7-methylguanosine occurred under the conditions used for oxidation and reduction. It was shown that the enzyme tobacco acid pyrophosphatase could be used to cleave cap dinucleotides containing unmodified or ring-opened ribose moieties and could also be used to release [3H]pm7G′ from NaB[3H]4-labeled rabbit globin mRNA. All five known cap 1 dinucleotides were resolved by hplc. The cap of rabbit globin mRNA was identified as m7Gpppm6Am, in agreement with other methods of determination. © 1979.

引用

页码：102 / 111

页数：10

共 32 条

[11] REOVIRUS MESSENGER-RNA CONTAINS A METHYLATED, BLOCKED 5'-TERMINAL STRUCTURE - M7G(5')PPP(5')GMPCP-
FURUICHI, Y
MORGAN, M
MUTHUKRISHNAN, S
SHATKIN, AJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (01) : 362 - 366
[12] GROSS HJ, 1975, MOL BIOL REP, V4, P105
[13] NATURE OF 5'-LINKED 5' NUCLEOTIDE-SEQUENCE AT 5' END OF RABBIT GLOBIN MESSENGER RIBONUCLEIC-ACID
HUNT, JA
OAKES, GN
[J]. BIOCHEMICAL JOURNAL, 1976, 155 (03) : 637 - 644
[14] INVIVO INHIBITION OF NOVIKOFF CYTOPLASMIC MESSENGER-RNA METHYLATION BY S-TUBERCIDINYL-HOMOCYSTEINE
KAEHLER, M
COWARD, J
ROTTMAN, F
[J]. BIOCHEMISTRY, 1977, 16 (26) : 5770 - 5775
[15] NUCLEOTIDE-SEQUENCE OF 5' TERMINUS OF ALFALFA MOSAIC-VIRUS RNA-4 LEADING INTO COAT PROTEIN CISTRON
KOPERZWARTHOFF, EC
LOCKARD, RE
ALZNEBDEWEERD, B
RAJBHANDARY, UL
BOL, JF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5504 - 5508
[16] KINETICS AND SPECIFICITY OF T4 POLYNUCLEOTIDE KINASE
LILLEHAUG, JR
KLEPPE, K
[J]. BIOCHEMISTRY, 1975, 14 (06) : 1221 - 1225
[17] SEQUENCE-ANALYSIS OF 5'[P-32] LABELED MESSENGER-RNA AND TRANSFER-RNA USING POLYACRYLAMIDE-GEL ELECTROPHORESIS
LOCKARD, RE
ALZNERDEWEERD, B
HECKMAN, JE
MACGEE, J
TABOR, MW
RAJBHANDARY, UL
[J]. NUCLEIC ACIDS RESEARCH, 1978, 5 (01) : 37 - 56
[18] NUCLEOTIDE-SEQUENCES AT 5' TERMINI OF RABBIT ALPHA AND BETA GLOBIN MESSENGER-RNA
LOCKARD, RE
RAJBHANDARY, UL
[J]. CELL, 1976, 9 (04) : 747 - 760
[19] METHYLATED CONSTITUENTS OF HETEROGENEOUS NUCLEAR-RNA - PRESENCE IN BLOCKED 5' TERMINAL STRUCTURES
PERRY, RP
KELLEY, DE
FRIDERICI, KH
ROTTMAN, FM
[J]. CELL, 1975, 6 (01) : 13 - 19
[20] METHYLATED CONSTITUENTS OF L CELL MESSENGER-RNA - EVIDENCE FOR AN UNUSUAL CLUSTER AT 5' TERMINUS
PERRY, RP
KELLEY, DE
FRIDERICI, K
ROTTMAN, F
[J]. CELL, 1975, 4 (04) : 387 - 394

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