SOLUTION CONFORMATION OF THE (-)-TRANS-ANTI-5-METHYLCHRYSENE-DG ADDUCT OPPOSITE DC IN A DNA DUPLEX - DNA BENDING ASSOCIATED WITH WEDGING OF THE METHYL-GROUP OF 5-METHYLCHRYSENE TO THE 3'-SIDE OF THE MODIFICATION SITE

This paper reports on NMR-molecular mechanics structural studies of the (-)-trans-anti[MC]dG adduct positioned opposite dC in the sequence context of the d(C1-C2-A3-T4-C5- [MC]G6-C7-T8-A9-C10-C11). d(G12-G13-T14-A15-G16-C17-G18-A19-T20-G21-G22 duplex [designated (-)-trans-anti-[MC]dG dC 11-mer duplex]. This adduct is derived from the trans addition at C-4 of (-)-anti-1(S),2(R)-dihydroxy- 3(R),4(S)-epoxy-1,2,3,4-tetrahydro-5-methylchlysene [(-)-anti-5-MeCDE] to the N-2 position of dG6 in this duplex sequence. The 5-methyl group is located adjacent to the MC(C-4) binding site, with these groups juxtaposed in a sterically crowded bay region in the adduct duplex. The 5-methylchrysene and the nucleic acid exchangeable and nonexchangeable protons were assigned following analysis of two-dimensional NMR data sets in H2O and D2O buffer solution. The solution structure of the (-)trans-anti-[MC]dG . dC 11-mer duplex has been determined by incorporating DNA-DNA and carcinogen-DNA proton-proton distances defined by lower and upper bounds deduced from NOESY data sets as restraints in molecular mechanics computations in torsion angle space. The results establish that the [MC]dG6 . dC17 base pair and flanking dC5 . dG18 and dC7 . dG16 base pairs retain Watson-Crick alignments upon adduct formation. The aromatic chrysenyl ring is positioned in the minor groove of a right-handed B-DNA helix and stacks predominantly over the sugar of the dC17 residue across from it on the unmodified complementary strand. The chrysenyl ring points toward the 3'-end of the modified strand with its 5-methyl group inserting between the modified [MC]dG6 . dC17 and dC7 . dG16 base pairs. The adduct duplex bends by similar to 47 degrees as a result of the wedged insertion of the 5-methyl group from the minor groove face of the duplex. The solution structure of the (-)-trans-anti-[MC]dG . dC 11-mer duplex is compared with that of the corresponding (-)-trans-anti-[BP]dG . dC 11-mer [De los Santos et al. (1992) Biochemistry 31, 5245-5252] in which the [BP]dG adduct is derived from the binding of (-)-anti-BPDE [7(S),8(R)-dihydroxy-9(R),10(S)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene to the N-2 position of dG in the same DNA sequence context. Although the solution structures of the (-)-trans-anti stereoisomers of 5-methylchrysenyl-dG and benzo[a]pyrenyl-dG adducts opposite dC exhibit many features in common with each other, the [MC]dG adduct which contains a bay region methyl group bends the DNA helix to a greater extent than in the corresponding [BP]dG adduct, which lacks a bay region methyl group. Carcinogen-induced bending effects may be important factors in the expression of the mutagenic potential of these [MC]dG lesions. Because of the stabilities of these lesions, site-directed mutagenesis studies are now feasible which should lead to new insights into the relationships between adduct structure, DNA structural distortions, and mutagenic specificity and activity.