DEGRADATION OF 2-CHLOROALLYLALCOHOL BY A PSEUDOMONAS SP

被引：20

作者：

VANDERWAARDE, JJ ^{[1
]}

KOK, R ^{[1
]}

JANSSEN, DB ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT BIOCHEM,NIJENBORGH 4,9747 AG GRONINGEN,NETHERLANDS

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 02期

关键词：

D O I：

10.1128/AEM.59.2.528-535.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three Pseudomonas strains capable of utilizing 2-chloroallylalcohol (2-chloropropenol) as the sole carbon source for growth were isolated from soil. The fastest growth was observed with strain JD2, with a generation time of 3.6 h. Degradation of 2-chloroallylalcohol was accompanied by complete dehalogenation. Chloroallylalcohols that did not support growth were dechlorinated by resting cells; the dechlorination level was highest if an alpha-chlorine substituent was present. Crude extracts of strain JD2 contained inducible alcohol dehydrogenase activity that oxidized mono- and dichloroallylalcohols but not trichloroallylalcohol. The enzyme used phenazine methosulfate as an artificial electron acceptor. Further oxidation yielded 2-chloroacrylic acid. The organism also produced hydrolytic dehalogenases converting 2-chloroacetic acid and 2-chloropropionic acid.

引用

页码：528 / 535

页数：8

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