SEGREGATION IN DNA SOLUTIONS INDUCED BY ELECTRIC-FIELDS

被引：79

作者：

MITNIK, L

HELLER, C

PROST, J

VIOVY, JL

机构：

[1] ECOLE SUPER PHYS & CHIM IND VILLE PARIS, CNRS, UA 1382, PHYSICOCHIM THEOR LAB, F-75231 PARIS 05, FRANCE

[2] ECOLE SUPER PHYS & CHIM IND VILLE PARIS, CNRS, UA 278, PHYSICOCHIM STRUCT & MACROMOLEC LAB, F-75231 PARIS 05, FRANCE

来源：

SCIENCE | 1995年 / 267卷 / 5195期

关键词：

D O I：

10.1126/science.7809626

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA solutions subjected to an electric field exhibit an instability that leads to DNA segregation in aggregates tilted with regard to the field. With the use of epifluorescence videomicroscopy, the evolution of DNA patterns in capillaries as a function of DNA concentration, DNA size, field strength, and field frequency was studied. The field threshold for segregation was decreased when the frequency was lowered or when the DNA molecular weight or concentration was increased. Aggregation is attributed to an electrohydrodynamic instability triggered by the dipole-dipole interaction. This phenomenon explains the failure of earlier attempts to separate large DNA in capillaries.

引用

页码：219 / 222

页数：4