EXPRESSION OF PROTEIN-KINASE-C ISOFORMS IN SMOOTH-MUSCLE CELLS IN VARIOUS STATES OF DIFFERENTIATION

被引：62

作者：

ASSENDER, JW ^{[1
]}

KONTNY, E ^{[1
]}

FREDHOLM, BB ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF,COLL MED,DEPT CARDIOL,CARDIFF,S GLAM,WALES

来源：

FEBS LETTERS | 1994年 / 342卷 / 01期

关键词：

PROTEIN KINASE C; DIFFERENTIATION; SMOOTH MUSCLE CELL;

D O I：

10.1016/0014-5793(94)80588-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analysed the expression of protein kinase C (PKC) isoforms in smooth muscle cells (SMC) in various states of differentiation, using Western blots and thermocycle amplification of mRNA. SMC isolated from intact tissues express three isoforms of PKC, namely alpha, delta, and zeta. Following cell culture, SMC additionally express mRNA for PKC-epsilon, but significant amounts of the corresponding protein were not detected. Transformed SMC, such as the cell line DDT1 MF-2, express both mRNA and protein for PKC-epsilon, lack the delta isoenyune, whilst maintaining the expression of the alpha and zeta isoforms. Thus PKC-delta and epsilon isoenzyme expression appears to vary with the state of differentiation of these cells, with PKC-epsilon expression increasing as the cells become proliferative.

引用

页码：76 / 80

页数：5

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