ANALYSIS OF ANGIOTENSIN-II RECEPTOR SUBTYPES IN INDIVIDUAL RAT-BRAIN NUCLEI

Previous studies have used new angiotensin II (AII) receptor subtype selective compounds to localize AII receptor subtypes within discrete rat brain nuclei. The purpose of this autoradiographic study was to extend these preliminary findings and provide a comprehensive analysis of AII binding sites in 22 rat brain nuclei and the anterior pituitary, to include estimates of the binding affinity for I-125 sar1 ile8 AII (I-125 SIAII) at each nucleus, and determine the fractional distribution of each subtype at each nucleus. Estimates of K(D) in separate experiments revealed that AT1 nuclei had a consistently higher affinity for I-125 SIAII than AT2 nuclei (0.66 vs. 2.55 nM). Displacement of subsaturating concentrations of I-125 SIAII by 10(-8)-10(-4) M DuP753 (selective for the AT1 subtype) or PD123177 (selective for the AT2 subtype) indicated that approximately half of the brain regions surveyed contained predominantly AT1 sites and half contained predominantly AT2 sites. Binding was partially displaced by both compounds in several regions and two site analyses were performed to estimate the distribution of subtypes within each nucleus. The data were then corrected for differential occupancy by I-125 SIAII. Brain nuclei associated with cardiovascular or dipsogenic actions of AII, e.g., subfornical organ, organum vasculosum of the lamina terminalis, median preoptic nucleus, nucleus of the solitary tract and area postrema, contained pure, or almost pure, populations of AT1 receptors. The functions of AII in brain regions containing predominantly AT2 binding sites, e.g., thalamus, colliculi, inferior olive and locus ceruleus, remain undefined. Thus, All binding sites in the rat brain have been differentiated into two subtypes with similar characteristics to those reported in peripheral tissues. However, the unexpected finding that they can be differentiated on the basis of their affinity for I-125 SIAII raises questions concerning their coidentity with peripheral receptor subtypes.