RESTRICTION ANALYSIS OF PHASE VARIATION IN XENORHABDUS SPP (ENTEROBACTERIACEAE), ENTOMOPATHOGENIC BACTERIA ASSOCIATED WITH NEMATODES

被引：33

作者：

AKHURST, RJ ^{[1
]}

SMIGIELSKI, AJ ^{[1
]}

MARI, J ^{[1
]}

BOEMARE, N ^{[1
]}

MOURANT, RG ^{[1
]}

机构：

[1] UNIV MONTPELLIER 2,INRA,PATHOL COMPAREE LAB,CNRS,F-34095 MONTPELLIER 05,FRANCE

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1992年 / 15卷 / 03期

关键词：

XENORHABDUS; X-NEMATOPHILUS; PHASE VARIATION; RFLP; METHYLATION; SOUTHERN CROSS; DNA REARRANGEMENT;

D O I：

10.1016/S0723-2020(11)80224-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

As part of an effort to elucidate the molecular basis of phase variation in Xenorhabdus, the two phases of X. nematophilus were examined for evidence of genomic rearrangements. No DNA sequence rearrangement was detected by RFLP analysis or Southern Cross hybridization of total DNA. Differential digestion efficiency by various restriction enzymes strongly indicated that the DNA of each of the Xenorhabdus species is highly methylated at both adenine and cytosine residues. The differential response to SmaI digestion by European and American isolates of X. nematophilus suggests a geographic variation in this species.

引用

页码：469 / 473

页数：5

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