LEUCINE SYNTHESIS IN SPINACH-CHLOROPLASTS - PARTIAL CHARACTERIZATION OF 2-ISOPROPYLMALATE SYNTHASE

被引：27

作者：

HAGELSTEIN, P ^{[1
]}

SCHULTZ, G ^{[1
]}

机构：

[1] TIERARZTL HSCH HANOVER, INST BOT, D-30559 HANNOVER, GERMANY

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1993年 / 374卷 / 12期

关键词：

CHLOROPLASTS; 2-ISOPROPYLMALATE SYNTHASE; LEUCINE SYNTHESIS; SPINACIA OLERACEA;

D O I：

10.1515/bchm3.1993.374.7-12.1105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2-isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The K-m values were 5 mu M for acetyl-CoA and 75 mu M for 2-oxoisovalerate.

引用

页码：1105 / 1108

页数：4

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