PLASMID-MEDIATED CONTROL OF NODULATION IN RHIZOBIUM-TRIFOLII

被引:59
作者
ZURKOWSKI, W
LORKIEWICZ, Z
机构
[1] Institute of Microbiology, University M. Curie-Sklodowska, Lublin, 20-033
关键词
Elimination of plasmid; Molecular weight of plasmid; Nodulation; Non-nodulating mutants; Plasmids; Rhizobium trifolii;
D O I
10.1007/BF00446820
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The nodulation ability was effectively eliminated from different Rhizobium trifolii strains incubated at elevated temperature (Żurkowski and Lorkiewicz, 1978). Non-nodulating (Nod - ) mutants were stable and no reversion of Nod - to Nod + phenotype was observed. Strains R. trifolii 24 and T12 which showed a high percentage of elimination of nodulation ability were examined in detail. Two plasmids were detected in strain 24 using neutral and alkaline sucrose gradient centrifugation of plasmid preparations. Molecular weights of the plasmids pWZ1 and pWZ2 were 460 Mdal and 190 Mdal, respectively. Rhizobium lysates labeled with 3 H-thymidine and ultracentrifuged in caesium chloride - ethidium bromide gradients demonstrated a 40% reduction of the plasmid DNA content in R. trifolii 24 Nod - mutants in comparison with the nodulating wild type strain 24. It was found further that non-nodulation of mutants 24 Nod - was due to the absence of plasmid pWZ2. Sucrose gradient data also demonstrated that strain T12 contained two plasmids with molecular weights corresponding to those of pWZ1 and pWZ2, respectively. In Nod - mutant clones derived from strain T12, pWZ2 plasmid was missing. © 1979 Springer-Verlag.
引用
收藏
页码:195 / 201
页数:7
相关论文
共 26 条
[1]   CHARACTERIZATION OF MULTIPLE CIRCULAR DNA FORMS OF COLINCINOGENIC FACTOR E1 FROM PROTEUS MIRABILIS [J].
BAZARAL, M ;
HELINSKI, DR .
BIOCHEMISTRY, 1968, 7 (10) :3513-&
[2]   PRESENCE OF EXTRACHROMOSOMAL DNA IN RHIZOBIUM-MELILOTI [J].
BECHET, M ;
GUILLAUME, JB .
CANADIAN JOURNAL OF MICROBIOLOGY, 1978, 24 (08) :960-966
[3]   MOLECULAR STRUCTURE OF BACTERIAL PLASMIDS [J].
CLOWES, RC .
BACTERIOLOGICAL REVIEWS, 1972, 36 (03) :361-+
[4]   TRANSMISSIBLE RESISTANCE TO PENICILLIN-G, NEOMYCIN, AND CHLORAMPHENICOL IN RHIZOBIUM-JAPONICUM [J].
COLE, MA ;
ELKAN, GH .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1973, 4 (03) :248-253
[5]  
DUNICAN LK, 1976, SYMBIOTIC NITROGEN F, P77
[7]   HIGH-FREQUENCY TRANSFER OF NODULATING ABILITY BETWEEN STRAINS AND SPECIES OF RHIZOBIUM [J].
JOHNSTON, AWB ;
BEYNON, JL ;
BUCHANANWOLLASTON, AV ;
SETCHELL, SM ;
HIRSCH, PR ;
BERINGER, JE .
NATURE, 1978, 276 (5688) :634-636
[8]   PHYSICAL EVIDENCE OF A PLASMID IN RHIZOBIUM-JAPONICUM [J].
KLEIN, GE ;
JEMISON, P ;
HAAK, RA ;
MATTHYSSE, AG .
EXPERIENTIA, 1975, 31 (05) :532-533
[9]  
KOWALCZUK E, 1977, ACTA MICROBIOL POL, V26, P9
[10]   EFFECT OF GROWTH AT ELEVATED TEMPERATURES ON SOME HERITABLE PROPERITIES OF STAPHYLOCOCCUS AUREUS [J].
MAY, JW ;
HOUGHTON, RH ;
PERRET, CJ .
JOURNAL OF GENERAL MICROBIOLOGY, 1964, 37 (02) :157-+