MOLECULAR-CLONING AND CHARACTERIZATION OF AN EXTRACELLULAR PROTEASE GENE FROM AEROMONAS-HYDROPHILA

被引：47

作者：

RIVERO, O ^{[1
]}

ANGUITA, J ^{[1
]}

PANIAGUA, C ^{[1
]}

NAHARRO, G ^{[1
]}

机构：

[1] UNIV LEON, FAC VET, UNIDAD MICROBIOL, DEPT PATOL ANIM SANIDAD ANIMAL, E-24071 LEON, SPAIN

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 07期

关键词：

D O I：

10.1128/jb.172.7.3905-3908.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A structural gene which codes for an extracellular protease in Aeromonas hydrophila SO2/2 and D13 was cloned in Escherichia coli C600-1 by using pBR322 as a vector. The gene codes for a temperature-stable protease with a molecular mass of approximately 38,000 daltons. The protein was secreted to the periplasm of E. coli C600-1 and purified by osmotic shock. Cloned protease (P3) was identical in molecular mass and properties to the one purified from A. hydrophila SO2/2 culture supernatant as an extracellular product.

引用

页码：3905 / 3908

页数：4

共 35 条

[1] EXTRACELLULAR VIRULENCE FACTORS OF AEROMONAS-HYDROPHILA IN FISH INFECTIONS [J].

ALLAN, BJ ;

STEVENSON, BMW .

CANADIAN JOURNAL OF MICROBIOLOGY, 1981, 27 (10) :1114-1122

[2] AMONABACTIN, A NOVEL TRYPTOPHAN-CONTAINING OR PHENYLALANINE-CONTAINING PHENOLATE SIDEROPHORE IN AEROMONAS-HYDROPHILA [J].

BARGHOUTHI, S ;

YOUNG, R ;

OLSON, MOJ ;

ARCENEAUX, JEL ;

CLEM, LW ;

BYERS, BR .

JOURNAL OF BACTERIOLOGY, 1989, 171 (04) :1811-1816

[3]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[4] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[5] CLONING, EXPRESSION, AND MAPPING OF THE AEROMONAS-HYDROPHILA AEROLYSIN GENE DETERMINANT IN ESCHERICHIA-COLI K-12 [J].

CHAKRABORTY, T ;

HUHLE, B ;

BERGBAUER, H ;

GOEBEL, W .

JOURNAL OF BACTERIOLOGY, 1986, 167 (01) :368-374

[6] MARKER EXCHANGE MUTAGENESIS OF THE AEROLYSIN DETERMINANT IN AEROMONAS-HYDROPHILA DEMONSTRATES THE ROLE OF AEROLYSIN IN A-HYDROPHILA-ASSOCIATED SYSTEMIC INFECTIONS [J].

CHAKRABORTY, T ;

HUHLE, B ;

HOF, H ;

BERGBAUER, H ;

GOEBEL, W .

INFECTION AND IMMUNITY, 1987, 55 (09) :2274-2280

[7]

DAHLE HK, 1971, ACTA PATH MICRO IM A, VB 79, P726

[8] PARTIAL-PURIFICATION OF THE EXTRACELLULAR PROTEOLYTIC SYSTEM OF AEROMONAS-HYDROPHILA LP50 - COMPARISON OF CHROMATOGRAPHIC AND ELECTROPHORETIC METHODS [J].

DENIS, F ;

VEILLETPONCET, L .

ANNALES DE MICROBIOLOGIE, 1984, A135 (02) :219-227

[9]

GROSS R, 1969, Australian Journal of Science, V31, P330

[10]

HENSEN C, 1980, ANAL BIOCHEM, V102, P196

← 1 2 3 4 →