DETECTION OF PENICILLIN AND EXTENDED-SPECTRUM CEPHALOSPORIN RESISTANCE AMONG STREPTOCOCCUS-PNEUMONIAE CLINICAL ISOLATES BY USE OF THE E-TEST

被引:115
作者
JORGENSEN, JH
FERRARO, MJ
MCELMEEL, ML
SPARGO, J
SWENSON, JM
TENOVER, FC
机构
[1] MASSACHUSETTS GEN HOSP,MICROBIOL LAB,BOSTON,MA 02114
[2] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,HOSP INFECT PROGRAM,ATLANTA,GA 30333
关键词
D O I
10.1128/JCM.32.1.159-163.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Increasing penicillin resistance and the initial recognition of resistance to extended-spectrum cephalosporins among Streptococcus pneumoniae isolates have placed greater emphasis on accurate methods for susceptibility testing of clinical isolates. This study has evaluated the use of the E test (AB Biodisk NA, Piscataway, N.J.) for the detection of penicillin and cefotaxime resistance among 147 pneumococcal clinical isolates in three geographically separate laboratories. These included 42 penicillin-resistant (MIC, greater-than-or-equal-to 2 mug/ml) and 14 cefotaxime-resistant (defined here as an MIC of greater-than-or-equal-to 2 mug/ml) isolates. E test strips were applied to the surface of Mueller-Hinton sheep blood agar plates and incubated at 35-degrees-C in 5% CO2 for 20 to 24 h. E test MICs were compared with MICs determined with lysed horse blood-supplemented Mueller-Hinton broth in a microdilution format as recommended by the National Committee for Clinical Laboratory Standards. Penicillin MICs agreed within one log2 dilution for 136 of 147 (92.5%) isolates, and cefotaxime MICs agreed within one log2 dilution for 142 of 147 (%.6%) isolates. No very major or major interpretive errors occurred with either penicillin or cefotaxime E test MIC results. There were 9.5 and 5.4% minor interpretive category errors with penicillin and cefotaxime E test MICs, respectively. These data indicate that the E test represents a convenient and reliable method for the detection of penicillin or cephalosporin resistance in pneumococci.
引用
收藏
页码:159 / 163
页数:5
相关论文
共 26 条