THE STRUCTURE OF A NOVEL POLYSACCHARIDE PRODUCED BY BRADYRHIZOBIUM SPECIES WITHIN SOYBEAN NODULES

被引:25
作者
AN, JH
CARLSON, RW
GLUSHKA, J
STREETER, JG
机构
[1] UNIV GEORGIA,COMPLEX CARBOHYDRATE RES CTR,ATHENS,GA 30602
[2] OHIO STATE UNIV,DEPT AGRON,WOOSTER,OH
关键词
BRADYRHIZOBIUM JAPONICUM; BRADYRHIZOBIUM ELKANII; NODULES; POLYSACCHARIDE;
D O I
10.1016/0008-6215(94)00361-I
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Certain strains of Bradyrhizobium japonicum and B. elkanii produce a polysaccharide within the root nodules of their legume host, soybean. These nodule polysaccharides (NPSs) were isolated and characterized. The NPS produced by B. elkanii strains proved to be identical in glycosyl composition and linkages to the extracellular polysaccharide (EPS) of this species indicating that the NPS and EPS for B. elkanii have identical structures (W.F. Dudman, Carbohydr. Res., 66 (1978) 9-23), [GRAPHICS] However, the structure of the NPS from B. japonicum proved to be quite different from that of its EPS. Methylation analysis of this NPS showed that it consists of 3-linked Gal, 3-linked Rha, 2,4-linked Rha, 4-linked Rha, and terminal 2-O-methyl GlcA in a 1:1:1:1:1 ratio. Stereochemical configurations of the glycosyl residues were determined by the preparation and analysis of trimethylsilyl (Me(3)Si) (-)-2-butyl glycosides. NMR spectroscopy (both H-1 and C-13) showed that the Gal residue is alpha-linked, while all the other glycosyl residues are beta-linked. Oligosaccharides produced by periodate oxidation-Smith degradation were purified, as were oligosaccharides produced by partial acid hydrolysis. Characterization of the Smith degradation products by methylation analysis, NMR spectroscopy, electrospray-mass spectrometry, and characterization of the partial acid hydrolysate oligosaccharides showed that the repeating oligosaccharide unit of the NPS has the structure, [GRAPHICS] CS]
引用
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页码:303 / 317
页数:15
相关论文
共 17 条
[11]   HIGH SELECTIVITY IN THE PARTIAL DEGRADATION OF AN EXTRACELLULAR POLYSACCHARIDE OF RHIZOBIUM-JAPONICUM WITH LIQUID-HYDROGEN FLUORIDE - A NMR-SPECTROSCOPIC STUDY [J].
MORT, AJ ;
UTILLE, JP ;
TORRI, G ;
PERLIN, AS .
CARBOHYDRATE RESEARCH, 1983, 121 (SEP) :221-232
[12]  
MORT AJ, 1982, J BIOL CHEM, V257, P1870
[13]   RECOVERY OF METHYLATED SACCHARIDES FROM METHYLATION REACTION MIXTURES USING SEP-PAK C18 CARTRIDGES [J].
MORT, AJ ;
PARKER, S ;
KUO, MS .
ANALYTICAL BIOCHEMISTRY, 1983, 133 (02) :380-384
[14]   COMPUTER-OPTIMIZED DECOUPLING SCHEME FOR WIDEBAND APPLICATIONS AND LOW-LEVEL OPERATION [J].
SHAKA, AJ ;
BARKER, PB ;
FREEMAN, R .
JOURNAL OF MAGNETIC RESONANCE, 1985, 64 (03) :547-552
[15]   FORMATION OF NOVEL POLYSACCHARIDES BY BRADYRHIZOBIUM-JAPONICUM BACTEROIDS IN SOYBEAN NODULES [J].
STREETER, JG ;
SALMINEN, SO ;
WHITMOYER, RE ;
CARLSON, RW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (02) :607-613
[16]  
York WS, 1985, METHOD ENZYMOL, P3, DOI DOI 10.1016/0076-6879(86)18062-1
[17]   A 2ND EXOPOLYSACCHARIDE OF RHIZOBIUM-MELILOTI STRAIN SU47 THAT CAN FUNCTION IN ROOT NODULE INVASION [J].
ZHAN, HJ ;
LEVERY, SB ;
LEE, CC ;
LEIGH, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (09) :3055-3059