APPLICATION OF CAPILLARY ZONE ELECTROPHORESIS FOR THE ANALYSIS OF PROTEINS, PROTEIN-SMALL MOLECULES, AND PROTEIN-DNA INTERACTIONS

被引：17

作者：

JANINI, GM

FISHER, RJ

HENDERSON, LE

ISSAQ, HJ

机构：

[1] NCI-Frederick Cancer Research and Development Center, SAIC Frederick, Frederick, Maryland, 21702, P.O. Box B

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 18-19期

关键词：

D O I：

10.1080/10826079508014614

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis with a polyacrylamide-coated capillary was used for the separation of proteins, and the study of protein-small molecule and protein-DNA interactions. The coating (10% T polyacrylamide) is of sufficient thickness and hydrophilicity to reduce protein adsorption and eliminate electroosmotic flow. Data on the effect of sample solvent constituents on the peak shape and position of protein solutes was investigated. The results show that CE is a relevant and fast technique for the study of protein-DNA and protein-drug interaction. Also, the addition of TRIS-buffer to the sample solution resulted in the CE focusing of some basic proteins, while others were not affected.

引用

页码：3617 / 3628

页数：12

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