SUBCELLULAR-DISTRIBUTION OF HEPATIC BILE ACID-CONJUGATING ENZYMES

被引：24

作者：

LIM, WC

JORDAN, TW

机构：

来源：

BIOCHEMICAL JOURNAL | 1981年 / 197卷 / 03期

关键词：

D O I：

10.1042/bj1970611

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The subcellular location of enzymes conjugating bile acids with glycine or taurine was investigated by centrifugation of rat liver homogenates. [14C]Cholic acid-conjugating activity was predominantly associated with the soluble-microsomal region of the gradient after centrifugation in a Ti-15 zonal rotor but the bulk of the conjugating activity sedimented with mitochondrial-lysosomal fractions in differential pelleting experiments. Cholate:CoA ligase (EC 6.2.1.7) and cholyltransferase (EC 2.3.1) were not enriched in purified Golgi or plasma-membrane fractions. Cholate:CoA ligase was distributed evenly between rough- and smooth-surfaced microsomal subfractions but cholyltransferase showed a dual soluble-rough microsomal activity distribution. Sedimentation of cholyltransferase in mitochondria-enriched fractions prepared by differential centrifugation appears to be an artefact of sedimentation of rough microsomal membranes in mitochondrial fractions. The subcellular distribution of bile acid-conjugating enzymes is discussed with reference to hepatic processing of bile acids.

引用

页码：611 / 618

页数：8

共 21 条

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