6-FLUOROPYRIDOXOL - A NOVEL PROBE OF CELLULAR PH USING F-19 NMR-SPECTROSCOPY

被引：19

作者：

MEHTA, VD ^{[1
]}

KULKARNI, PV ^{[1
]}

MASON, RP ^{[1
]}

CONSTANTINESCU, A ^{[1
]}

ARAVIND, S ^{[1
]}

GOOMER, N ^{[1
]}

ANTICH, PP ^{[1
]}

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT RADIOL,DALLAS,TX 75235

来源：

FEBS LETTERS | 1994年 / 349卷 / 02期

关键词：

F-19; NMR; PH; FLUOROPYRIDOXOL; BLOOD;

D O I：

10.1016/0014-5793(94)00675-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (similar to 10 ppm acid/base shift) and a pK(a) similar to 8.2 appropriate for physiological investigations. Using F-19 NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and P-31 NMR spectroscopy.

引用

页码：234 / 238

页数：5

共 27 条

[1] HEPATIC INTRACELLULAR PH INVIVO USING F-QUENE-1 AND F-19 NMR-SPECTROSCOPY [J].