XYLANASE ENCODED BY GENETICALLY-ENGINEERED CLOSTRIDIUM-THERMOCELLUM GENE IN BACILLUS-SUBTILIS

被引：4

作者：

CHO, KH

JUNG, KH

PACK, MY

机构：

[1] KOREA ADV INST SCI & TECHNOL, DEPT BIOTECHNOL, TAEJON 305701, SOUTH KOREA

[2] MIT, DEPT BIOL, CAMBRIDGE, MA USA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 02期

关键词：

D O I：

10.1007/BF00127980

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Xylanase was produced with Bacillus subtilis (pJXI8), constructed previously, which contains Clostridium thermocellum xylanase gene expressing with a strong Bacillus promoter. The enzyme hydrolyzed oat spelt xylan to mostly xylobiose and xylotriose which are preferred for industrial applications. The optimal temperature and pH for the activity of this enzyme were 60-degrees-C and 5.4, respectively, with moderate stability under these conditions.

引用

页码：157 / 160

页数：4

共 10 条

[1]

ABE K, 1986, Patent No. 242592

[2] PURIFICATION AND PROPERTIES OF EXTRACELLULAR ENDOXYLANASES FROM ALKALOPHILIC THERMOPHILIC BACILLUS SP [J].