TPA CAUSES DIVERGENT RESPONSES OF CA2+-DEPENDENT AND CA2+-INDEPENDENT ISOFORMS OF PKC IN THE NUCLEI OF CACO-2 CELLS

被引：18

作者：

FRAWLEY, BP ^{[1
]}

TIEN, XY ^{[1
]}

HARTMANN, SC ^{[1
]}

WALI, RK ^{[1
]}

NIEDZIELA, SM ^{[1
]}

DAVIDSON, NO ^{[1
]}

SITRIN, MD ^{[1
]}

BRASITUS, TA ^{[1
]}

BASSONNETTE, M ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT MED,GI SECT,CHICAGO,IL 60637

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1222卷 / 02期

关键词：

PHORBOL ESTER; SIGNAL TRANSDUCTION; NUCLEAR PROTEIN KINASE C;

D O I：

10.1016/0167-4889(94)90182-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present studies were undertaken to examine the expression of PKC isoforms within the nucleus of Caco-2 cells, a cell line widely used to investigate intestinal cell growth and differentiation, in order to begin to explore their roles in modulating gene expression. Purified nuclei were, therefore, prepared from Caco-2 cells and found to contain PKC-zeta, but not -alpha. The phorbol ester, 12-O-tetradecanoyl phorbol 13-acetate (TPA) caused an acute redistribution of PKC-alpha to the nucleus, but did not change the distribution of PKC-zeta. Chronic treatment with TPA down-regulated total PKC-alpha, but not -zeta. Moreover, in contrast to acute TPA treatment, after chronic treatment, nuclear PKC-alpha was no longer detectable, whereas nuclear PKC-zeta was unchanged. These studies demonstrate for the first time the constitutive expression and divergent responses to TPA of the Ca2+-dependent and Ca2+-independent isoforms of PKC in the nuclei of Caco-2 cells and suggest that these specific isoforms may be involved in modulating gene expression.

引用

页码：301 / 305

页数：5

共 26 条

[11] ZETA-RELATED PROTEIN KINASE-C IN NUCLEI OF NERVE-CELLS [J].