CYCLOSPORINE INCREASES GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) ACTIVITY AND GENE-EXPRESSION IN MURINE KERATINOCYTES

被引:14
作者
GALLO, RL
GRABBE, S
CHOI, SS
BLEICHER, P
GRANSTEIN, RD
机构
[1] MGH-Harvard Cutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital East, Charlestown, MA
关键词
D O I
10.1111/1523-1747.ep12497858
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Keratinocytes produce multiple cytokines in response to a variety of stimuli. The release of interleukin 1 (IL-1) from keratinocytes may be significant in initiation of cutaneous inflammation, and the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF) is thought to be important in the regulation of antigen-presenting function by epidermal Langerhans cells. Because cyclosporin inhibits interleukin 2 release from T cells, it has been suggested that cyclosporin may function as an anti-inflammatory agent within the epidermis through inhibition of keratinocyte cytokine release. This investigation examined the direct effect of cyclosporin on the production of GM-CSF by murine keratinocytes and the keratinocyte cell line PAM 212. GM-CSF bioactivity increased in cell supernatants from keratinocytes exposed in vitro to 1-mu-g/ml cyclosporin for up to 24 h. GM-CSF and IL-1 mRNA levels in keratinocytes cultured under similar conditions or in the presence of lipopolysaccharide also increased. The lack of inhibition of GM-CSF expression following cyclosporin treatment is consistent with recent observations in T cells and is opposite to the effect of cyclosporin on interleukin 2.
引用
收藏
页码:274 / 278
页数:5
相关论文
共 36 条
[11]   GROWTH OF FACTOR-DEPENDENT HEMATOPOIETIC PRECURSOR CELL-LINES [J].
DEXTER, TM ;
GARLAND, J ;
SCOTT, D ;
SCOLNICK, E ;
METCALF, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 1980, 152 (04) :1036-1047
[12]  
DUELL EA, 1987, J INVEST DERMATOL, V88, P486
[13]  
ELDER JT, 1989, SCIENCE, V243, P911
[14]   NUCLEOTIDE-SEQUENCE OF THE HUMAN GAMMA-CYTOSKELETAL ACTIN MESSENGER-RNA - ANOMALOUS EVOLUTION OF VERTEBRATE NONMUSCLE ACTIN GENES [J].
ERBA, HP ;
GUNNING, P ;
KEDES, L .
NUCLEIC ACIDS RESEARCH, 1986, 14 (13) :5275-5294
[15]   THE EFFECT OF CYCLOSPORIN-A ON EPIDERMAL-CELLS .2. CYCLOSPORIN-A INHIBITS PROLIFERATION OF NORMAL AND TRANSFORMED KERATINOCYTES [J].
FURUE, M ;
GASPARI, AA ;
KATZ, SI .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 90 (06) :796-800
[16]   REGULATION OF GM-CSF AND IL-3 PRODUCTION FROM THE MURINE KERATINOCYTE CELL-LINE PAM-212 FOLLOWING EXPOSURE TO ULTRAVIOLET-RADIATION [J].
GALLO, RL ;
STASZEWSKI, R ;
SAUDER, DN ;
KNISELY, TL ;
GRANSTEIN, RD .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (02) :203-209
[17]  
Gallo RL, 1989, SKIN IMMUNE SYSTEM, P381
[18]  
KAHAN BD, 1989, NEW ENGL J MED, V321, P1725
[19]  
KIRNBAUER R, 1989, J IMMUNOL, V142, P1922
[20]   TUMOR NECROSIS FACTOR-ALPHA MAINTAINS THE VIABILITY OF MURINE EPIDERMAL LANGERHANS CELLS IN CULTURE, BUT IN CONTRAST TO GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR, WITHOUT INDUCING THEIR FUNCTIONAL MATURATION [J].
KOCH, F ;
HEUFLER, C ;
KAMPGEN, E ;
SCHNEEWEISS, D ;
BOCK, G ;
SCHULER, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (01) :159-171