IDENTIFICATION OF A NOVEL LARGE EXTRACHROMOSOMAL DNA (LED) IN THE TRYPANOSOMATIDAE

被引：9

作者：

WAGNER, W

SO, M

机构：

[1] Department of Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon, 97201-3098

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 16期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01405.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified a novel 75 kbp large extrachromosomal DNA (LED) which is stably maintained during developmental conversion of Trypanosoma cruzi. It has a covalently closed circular conformation and is not derived from the kinetoplast network. In all T. cruzi strains analysed, LED contains 18S rRNA and spliced leader (sl) sequences. LED from the T. cruzi Y strain contains a minimum of 15 copies of the sl repeat arrayed in a head-to-tail configuration and 50 copies of a 196 bp repeat. LED is also present in Trypanosoma dionisii (subgenus Schizotrypanosoma) and in other members of the family Trypanosomatidae. LED from different T. cruzi strains and from other members of the Trypanosomatidae differ in their content of large ribosomal subunit rRNA sequences and the 196 bp repeat. The presence of LED in four evolutionarily distant trypanosomatid species suggests that it plays an important role in the biology of these parasites.

引用

页码：2299 / 2308

页数：10

共 48 条

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