PRIMARY STRUCTURE DETERMINATION OF MONOACYLGLYCEROL AND DIACYLGLYCEROL LIPASE FROM PENICILLIUM-CAMEMBERTII

被引：15

作者：

ISOBE, K ^{[1
]}

NOKIHARA, K ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,DIV ENZYME TECHNOL & NAT PROD CHEM,W-3300 BRAUNSCHWEIG,GERMANY

来源：

FEBS LETTERS | 1993年 / 320卷 / 02期

关键词：

PENICILLIUM-CAMEMBERTII LIPASE; AMINO ACID SEQUENCE; PRIMARY STRUCTURE; PEPTIDE MAPPING; INTERNAL SEQUENCE ANALYSIS; 2-DIMENSIONAL ELECTROPHORESIS;

D O I：

10.1016/0014-5793(93)80071-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of mono- and diacylglycerol lipase from Penicillium camembertii was determined. This lipase has a single polypeptide chain consisting of 276 amino acid residues with two disulfide linkages. The primary structure was revealed by sequencing the digests of the intact and S-pyridylethylated proteins by trypsin, endoproteinase Lys-C and V8 protease. The two-dimensional electrophoresis was also carried out to confirm the internal sequence. The catalytic triad of this lipase was Ser, Asp and His, and one potential N-glycosylation site was also revealed.

引用

页码：101 / 106

页数：6

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