CLASS-II GENES OF MINIATURE SWINE .2. MOLECULAR-IDENTIFICATION AND CHARACTERIZATION OF B-(BETA) GENES FROM THE SLAC HAPLOTYPE

被引:23
作者
PRATT, K [1 ]
SACHS, DH [1 ]
GERMANA, S [1 ]
ELGAMIL, M [1 ]
HIRSCH, F [1 ]
GUSTAFSSON, K [1 ]
LEGUERN, C [1 ]
机构
[1] NCI,IMMUNOL BRANCH,TRANSPLANTAT BIOL SECT,BLDG 10,ROOM 4B13,BETHESDA,MD 20892
关键词
D O I
10.1007/BF00702482
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Genomic clones corresponding to class II β genes of the SLAc haplotype of miniature swine have been isolated and characterized. These genes have been grouped into seven non-overlapping clusters on the basis of restriction mapping. Ordering of exons within each cluster was accomplished by hybridization of Southern blots of restriction fragments with exon-specific probes. The two clusters (clusters 2 and 3) encoding the DRB and DQB genes were identified on the basis of hybridization with locus-specific 3′ untranslated cDNA probes. Cluster 4 contained exons of both DOB and DQB genes, the basis for which remains to be determined. The remaining four clusters (1, 5, 6, 7) were identified as containing DP, DR, and DO coding sequences, respectively, on the basis of sequence analysis. The porcine class II region appears very similar to that of man in number and nature of the class II genes identified and in the intron/exon organization of corresponding genes. © 1990 Springer-Verlag.
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页码:1 / 6
页数:6
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