PURIFICATION AND PROPERTIES OF BETA-N-ACETYLHEXOSAMINIDASE-A FROM PIG BRAIN

被引：5

作者：

GARCIAALONSO, J ^{[1
]}

REGLERO, A ^{[1
]}

CABEZAS, JA ^{[1
]}

机构：

[1] UNIV SALAMANCA,FAC BIOL,DEPT BIOCHEM & MOLEC BIOL,SALAMANCA,SPAIN

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1990年 / 22卷 / 06期

关键词：

D O I：

10.1016/0020-711X(90)90043-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Adult pig brain β-N-acetylhexosaminidase was separated into four different forms by ion exchange chromatography on diethylaminoethyl-cellulose. 2. 2. Form A was purified 1300-1500 fold by an unusual procedure, the technique of ampholyte displacement, followed by chromatography on concanavalin A Sepharose. 3. 3. The enzyme catalyses the hydrolysis of both β-N-acetylglucosaminides and β-N-acetylgalactosaminides. 4. 4. The kinetic studies support the evidence of the association of both activities to a single protein, and at the same active site. 5. 5. A natural substrate, N,N′-diacetylchitobiose, is also hydrolyzed, but not ovalbumin. 6. 6. This enzyme may be considered as an exoglycosidase. © 1990.

引用

页码：645 / 651

页数：7

共 37 条

[1] STUDIES ON HUMAN N-ACETYL-BETA-D-HEXOSAMINIDASE-C SEPARATED FROM NEONATAL BRAIN [J].

BESLEY, GTN ;

BROADHEAD, DM .

BIOCHEMICAL JOURNAL, 1976, 155 (01) :205-208

[2] CATABOLISM OF N-GLYCOSYLPROTEIN GLYCANS - EVIDENCE FOR A DEGRADATION PATHWAY OF SIALYLGLYCO-ASPARAGINES RESULTING FROM THE COMBINED ACTION OF THE LYSOSOMAL ASPARTYLGLUCOSAMINIDASE AND ENDO-N-ACETYL-BETA-D-GLUCOSAMINIDASE - A 400-MHZ H-1-NMR STUDY [J].

BRASSART, D ;

BAUSSANT, T ;

WIERUSZESKI, JM ;

STRECKER, G ;

MONTREUIL, J ;

MICHALSKI, JC .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 169 (01) :131-136

[3]

BRUNNGRABER EG, 1975, NEUROBIOLOGY, V5, P339

[4]

BRUNNGRABER EG, 1972, FUNCTIONAL STRUCTURA, P109

[5] PURIFICATION AND PROPERTIES OF BETA-N-ACETYLHEXOSAMINIDASE FROM MOLLUSK HELICELLA-ERICETORUM MULLER [J].

CALVO, P ;

REGLERO, A ;

CABEZAS, JA .

BIOCHEMICAL JOURNAL, 1978, 175 (02) :743-750

[6] THE DETERMINATION OF ENZYME INHIBITOR CONSTANTS [J].

DIXON, M .

BIOCHEMICAL JOURNAL, 1953, 55 (01) :170-171

[7]

DIXON M, 1979, ENZYMES, P886

[8] ENZYMATIC HYDROLYSIS OF SPHINGOLIPIDS .6. HYDROLYSIS OF CERAMIDE GLYCOSIDES BY CALF BRAIN BETA-N-ACETYLHEXOSAMINIDASE [J].

FROHWEIN, YZ ;

GATT, S .

BIOCHEMISTRY, 1967, 6 (09) :2783-&

[9]

GARCIA MV, 1980, INT J BIOCHEM, V13, P179

[10] DIFFERENTIAL DETERMINATION OF HEXOSAMINIDASES A AND B AND OF 2 FORMS OF BETA-GALACTOSIDASE, IN LAYERS OF HUMAN CEREBELLUM [J].

HIRSCH, HE .

JOURNAL OF NEUROCHEMISTRY, 1972, 19 (06) :1513-&

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