DOWN-MODULATION OF C-KIT MESSENGER-RNA AND PROTEIN EXPRESSION BY ERYTHROID-DIFFERENTIATION FACTOR/ACTIVIN-A

被引：14

作者：

HINO, M

NISHIZAWA, Y

TATSUMI, N

TOJO, A

MORII, H

机构：

[1] OSAKA CITY UNIV,SCH MED,DEPT INTERNAL MED 2,ABENO KU,OSAKA 545,JAPAN

[2] UNIV TOKYO,INST MED SCI,DEPT HEMATOL ONCOL,MINATO KU,TOKYO 108,JAPAN

来源：

FEBS LETTERS | 1995年 / 374卷 / 01期

关键词：

C-KIT; ERYTHROLEUKEMIA; EDF/ACTIVIN A; DIFFERENTIATION;

D O I：

10.1016/0014-5793(95)01078-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the effects of erythroid differentiation factor (EDF)/activin A on the expression of c-kit mRNA and protein in murine erythroleukemia (MEL) cells, EDF/activin A induced MEL cells to benzidine positive cells. Northern blot analysis showed that the c-kit mRNA expression was reduced synchronously with increase of beta-globin and uroporphyrinogen decarboxylase gene expression during EDF/activin A induced erythroid differentiation. Scatchard analysis indicated that the cell surface receptor number was reduced without change of affinity during differentiation. Our results suggest that EDF/activin A may act as a natural regulator of erythropoiesis with modulation of c-kit gene expression.

引用

页码：69 / 71

页数：3

共 29 条

[1] MOLECULAR-CLONING OF MAST-CELL GROWTH-FACTOR, A HEMATOPOIETIN THAT IS ACTIVE IN BOTH MEMBRANE-BOUND AND SOLUBLE FORMS [J].