DOWN-MODULATION OF C-KIT MESSENGER-RNA AND PROTEIN EXPRESSION BY ERYTHROID-DIFFERENTIATION FACTOR/ACTIVIN-A

被引：14

作者：

HINO, M

NISHIZAWA, Y

TATSUMI, N

TOJO, A

MORII, H

机构：

[1] OSAKA CITY UNIV,SCH MED,DEPT INTERNAL MED 2,ABENO KU,OSAKA 545,JAPAN

[2] UNIV TOKYO,INST MED SCI,DEPT HEMATOL ONCOL,MINATO KU,TOKYO 108,JAPAN

来源：

FEBS LETTERS | 1995年 / 374卷 / 01期

关键词：

C-KIT; ERYTHROLEUKEMIA; EDF/ACTIVIN A; DIFFERENTIATION;

D O I：

10.1016/0014-5793(95)01078-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the effects of erythroid differentiation factor (EDF)/activin A on the expression of c-kit mRNA and protein in murine erythroleukemia (MEL) cells, EDF/activin A induced MEL cells to benzidine positive cells. Northern blot analysis showed that the c-kit mRNA expression was reduced synchronously with increase of beta-globin and uroporphyrinogen decarboxylase gene expression during EDF/activin A induced erythroid differentiation. Scatchard analysis indicated that the cell surface receptor number was reduced without change of affinity during differentiation. Our results suggest that EDF/activin A may act as a natural regulator of erythropoiesis with modulation of c-kit gene expression.

引用

页码：69 / 71

页数：3

共 29 条

[21] THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS [J].

SCATCHARD, G .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1949, 51 (04) :660-672

[22] PURIFICATION AND CHARACTERIZATION OF AN FSH RELEASING PROTEIN FROM PORCINE OVARIAN FOLLICULAR-FLUID [J].

VALE, W ;

RIVIER, J ;

VAUGHAN, J ;

MCCLINTOCK, R ;

CORRIGAN, A ;

WOO, W ;

KARR, D ;

SPIESS, J .

NATURE, 1986, 321 (6072) :776-779

[23] MODULATION OF C-KIT MESSENGER-RNA AND PROTEIN BY HEMATOPOIETIC GROWTH-FACTORS [J].

WELHAM, MJ ;

SCHRADER, JW .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (05) :2901-2904

[24] IDENTIFICATION OF A LIGAND FOR THE C-KIT PROTOONCOGENE [J].

WILLIAMS, DE ;

EISENMAN, J ;

BAIRD, A ;

RAUCH, C ;

VANNESS, K ;

MARCH, CJ ;

PARK, LS ;

MARTIN, U ;

MOCHIZUKI, DY ;

BOSWELL, HS ;

BURGESS, GS ;

COSMAN, D ;

LYMAN, SD .

CELL, 1990, 63 (01) :167-174

[25]

YAMASHITA T, 1992, BLOOD, V79, P304

[26] HUMAN PROTOONCOGENE C-KIT - A NEW CELL-SURFACE RECEPTOR TYROSINE KINASE FOR AN UNIDENTIFIED LIGAND [J].

YARDEN, Y ;

KUANG, WJ ;

YANGFENG, T ;

COUSSENS, L ;

MUNEMITSU, S ;

DULL, TJ ;

CHEN, E ;

SCHLESSINGER, J ;

FRANCKE, U ;

ULLRICH, A .

EMBO JOURNAL, 1987, 6 (11) :3341-3351

[27]

YU J, 1989, BLOOD, V73, P952

[28]

YU J, 1987, NATURE, V300, P765

[29] STEM-CELL FACTOR IS ENCODED AT THE SI-LOCUS OF THE MOUSE AND IS THE LIGAND FOR THE C-KIT TYROSINE KINASE RECEPTOR [J].

ZSEBO, KM ;

WILLIAMS, DA ;

GEISSLER, EN ;

BROUDY, VC ;

MARTIN, FH ;

ATKINS, HL ;

HSU, RY ;

BIRKETT, NC ;

OKINO, KH ;

MURDOCK, DC ;

JACOBSEN, FW ;

LANGLEY, KE ;

SMITH, KA ;

TAKEISHI, T ;

CATTANACH, BM ;

GALLI, SJ ;

SUGGS, SV .

CELL, 1990, 63 (01) :213-224

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