IMMUNOFLUORESCENT STAINING FOR FACTOR-VIII-RELATED ANTIGEN - TOOL FOR STUDY OF HEALING IN VASCULAR PROSTHESES

被引：21

作者：

DILLEY, R

HERRING, M

BOXER, L

GARDNER, A

GLOVER, J

机构：

[1] INDIANA UNIV,MED CTR,DEPT PEDIAT,INDIANAPOLIS,IN 46202

[2] INDIANA UNIV,MED CTR,DEPT SURG,INDIANAPOLIS,IN 46202

[3] ST VINCENTS HOSP,DEPT SURG,INDIANAPOLIS,IN 46208

来源：

JOURNAL OF SURGICAL RESEARCH | 1979年 / 27卷 / 03期

关键词：

D O I：

10.1016/0022-4804(79)90124-0

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Localization of Factor VIII-related antigen by indirect immunofluorescent microscopy reliably identifies endothelium in frozen sections of canine blood vessels, including vasa vasorum. Using this technique, endothelium was found to line 6-cm-long porous Dacron arterial grafts in place in dogs for 6 months and 1 year. Grafts seeded with autogenous, venous endothelial cells at implantation showed specific fluorescence in the midportions of their flow surfaces after 1 month. Unseeded grafts were devoid of specific fluorescence beyond the region of pannus ingrowth. Vasa vasorum were very well delineated, even when no more than a row of single cells. We conclude that localization of Factor VIII-related antigen reliably identifies true endothelium in vascular grafts and will be useful in the study of vascular prosthetic healing. © 1979.

引用

页码：149 / 155

页数：7

共 20 条

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